Combination Magnesium and Insulin Leaf Supplements on Malondialadehid Levels and Proteins Profile in White Rats (Rattus Norvegicus) -Induced Streptozotocin

Abstract

Chronic hyperglycemia is a hallmark of diabetes mellitus (DM), a metabolic condition caused by decreased insulin hormone secretion, insulin receptor sensitivity, or both. Oxidative stress conditions that can raise the risk of micro and macrovascular problems can be brought on by hyperglycemia. The purpose of the study was to estimate the percentage reduction in malondialdehyde (MDA) levels and protein profile in white rats that had been given streptozotocin (STZ)-induced levels of MDA. A total of 40 male Wistar rats, weighing between 170 and 200 g, were used in this experimental type of study, and they were maintained in a healthy state for 7 days. Then injected with STZ 55 mg/Kg BW intraperitoneally to condition DM for 3 days, and 40 rats were divided into 10 group, K- as a negative control, K+ positive control who were treated with metformin 500 mg, K1 and K2 were given Mg 150 and 300 mg/day kgBB; K3 and K4 were given DI 75 and 300 mg/kgBW; and P1, P2, P3 and P4 were given various combinations of Mg:Insulin leaves 150:75; 150:300; 300:75; 300:300 mg/kgBB for 28 days. Results: The findings of this investigation demonstrated that the therapy group, which used a combined dose of magnesium supplement 150 mg/kg BW and insulin leaf 300 mg/kg BW, achieved the largest decrease in MDA levels in the livers of DM rats of 87.72%. Between the healthy rat group, the DM rat group, and the combination of doses of magnesium supplements and insulin leaves, proteins profile in the kidney organ of DM rats showed similarities and differences. Conclussion. In DM rats, a combination of insulin leaf 300 mg/kg BW and Supplement Mg 150 mg/kg BW can be utilized as an anti-diabetic medication.