Reducing Chemical Oxygen Demand, Total Coliform, and Escheria coli levels at the UPTD Health Laboratory in Kudus district using ZSM-5/TiO2 with TiO2 Mass Variations

Abstract

The Kudus health laboratory is a laboratory that carries out health examination services, due to several activities in the laboratory (the use of chemicals in the Hematology laboratory, Clinical Chemistry, and Health and Environmental Chemistry and handling of samples produces liquid waste). Liquid waste is hazardous material containing bacteria, viruses and chemical compounds. One of the parameters of Chemical Oxygen Demand (COD) number contamination with COD threshold value according to the Regional Regulation of Central Java Province No. 5 of 2012, namely 80mg /L. The aim of this research is to reduce COD levels, the number of Coliforms and E. coli in wastewater at the Kudus Regency Health Laboratory UPTD with mass ratio of dengan ZSM-5:TiO2 20:1, 20:3, 20:5 and 20:7 and characterization using  X-Ray Diffraction and Scanning Electrone Microscope. This research is an experimental research supported by literature study with variations in the ratio of Zeolite ZSM-5-TiO2 20: 1, 20: 3, 20: 5, and 20: 7 and the duration of immersion for 30 minutes. The results of this study showed that the percentage reduction in COD levels after soaking ZSM-5-TiO2 zeolite powder with variations in the ratio of 20: 1, 20: 3, 20: 5, and 20: 7 with 30 minutes of soaking time respectively increased from 25.51; 29.82; 42.58; and 55.34%.  The best percentage reduction in the number of Coliforms and E. coli was obtained in the ZSM-5/TiO₂ powder 20:1, of Coliforms decreased by 99.57% and the number of E. coli decreased by 100%. Conclussion: The best percentage reduction in COD was obtained in the ZSM-5/TiO₂ powder 20:7 and number of Coliforms and E. coli were obtained in the ZSM-5/TiO₂ powder 20:7.